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¹ÙÀÌ·¯½ºÇÐ Trizol reagent method for RNA extraction ½ÇÇè¿¡ ´ëÇÑ °á°ú º¸°í¼. ÀÌ ½ÇÇèÀº RT-PCRÀ» ÇϱâÀ§Çؼ Trizol reagent method¸¦ ÀÌ¿ëÇØ poliovirusÀÇ RNA¸¦ ºÐ¸®Çϰí Á¤Á¦ÇÏ´Â ½ÇÇèÀ¸·Î º»¹®Àº ½ÇÇè ¸ñÇ¥, ¿ø¸®, °á°ú»çÁø, ½ÇÇè ¹æ¹ý ¼³¸í, RNA ±¸Á¶ÀÇ ºÒ¾ÈÁ¤¼º, RNas ¿À¿° ¹× Á¦°ÅÆ÷ÇÔ
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1. Title
2. Objective
3. Principle
4. Method
5. Result
6. Discussion
7. References
º»¹®/³»¿ë
1. Title : Trizol reagent method for RNA extraction
2. Objective : poliovirus·Î RNA¸¦ Trizol reagent method¸¦ ÀÌ¿ëÇØ ÃßÃâÇϰí, RT-PCRÀ» ÀÌ¿ëÇØ cDNA·Î ¸¸µç µÚ, PCRÀ» ÀÌ¿ëÇØ ÁõÆø½ÃŲ´Ù.
3. Principle : RT-PCRÀ» ÇϱâÀ§ÇÑ poliovirusÀÇ RNA¸¦ ¾ò±âÀ§Çؼ Trizol reagent method¸¦ ÀÌ¿ëÇØ poliovirusÀÇ RNA¸¦ ºÐ¸®Çϰí Á¤Á¦ÇÑ´Ù.
4. Method
1) Phase seperation : RNA ºÐ¸®
¨ç Add 0.8ml of Trizol reagent per 0.2ml of sample.
¡æ Trizol reagent¿¡ Æ÷ÇÔµÈ Æä³îÀº Èֹ߼ºÀÌ ³ôÀº ÀÎü¿¡ À¯ÇØÇÑ µ¶¼º¹°ÁúÀ̹ǷΠ³¿»õ¸¦ ¸ÃÁö ¾Ê°í Á¶½ÉÈ÷ ´Ù·é´Ù.
¨è Vortexing 15sec X3 times.
¨é Incubate 5min, RT
Âü°í¹®Çå
³²»ó¿í ¿Ü 2ÀÎ/ À¯Àü°øÇÐÀÇ ÀÌÇØ/ ¶óÀÌÇÁ »çÀ̾ð½º/ p.44~46
https://assets.fishersci.com/TFS-Assets/LSG/manuals/MAN0013149_Glycogen_RNA_grade_UG.pdf