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Title : The Mechanisms of Stomatal Opening
�뼚 �꽌濡� :
1. 利앹궛怨� 愿묓빀�꽦
�떇臾쇱쓽 �쑀湲곕Ъ��� ���湲곗쓽 CO2濡쒕���꽣 湲곗썝�븯誘�濡� �씠瑜� �쐞�븯�뿬 CO2瑜� 異⑸텇�엳 諛쏆븘 �뱾�뿬�빞留� �븳�떎. �씠�븣 �옄�떊�씠 �븘�슂濡� �븯�뒗 臾쇱쓽 �쑀�떎��� 理쒕���븳 諛⑹���븯�뿬�빞 �븯硫� 愿묓빀�꽦怨� �샇�씉 �궗�씠�쓽 議곗젅�씠 �븘�슂�븯�떎. �씠瑜� �쐞�빐 議댁옱�븯�뒗 �듅�닔 援ъ“臾쇱씠 stomatal complex�씠�떎.
2. 湲곌났 (Stomatal complex)�쓽 援ъ“
1) 遺��꽭�룷 (Subsidiary cell): 怨듬���꽭�룷�뿉 �씤�젒�븯�뿬 湲곌났�쓽 媛쒗룓�뿉 �쁺�뼢�쓣 以��떎. �떇臾� 醫낆뿉 �뵲�씪 �떎�뼇�븳 �닔媛� 議댁옱�븯硫�, �뾾�쓣 �닔�룄 �엳�떎.
2) 怨듬�� �꽭�룷 (Guard cell): �뿽濡앹껜媛� 議댁옱�븯硫� 愿묓빀�꽦�룄 �씪�뼱�궃�떎. 臾쇱쓽 �넀�떎�쓣 諛⑹���븯湲� �쐞�븯�뿬 hair媛� 諛쒕떖�릺 �굹 �몴�뵾 �븞�쑝濡� �븿紐곕맂 援ъ“瑜� �씠猷④린�룄 �븳�떎. 怨듬���꽭�룷�뒗 �꽭�룷踰쎌쓽 �븳履쎌씠 紐⑺솕 �릺�뼱 �엳怨� �꽭�룷踰쎌쓽 援ъ“臾쇱씤 ���猷곕줈�뒪 �꽟�쑀媛� 諛⑹궗�긽�쑝濡� 諛곗뿴�릺�뼱�엳�뒗 �듅吏뺤쓣 吏��땲怨� �엳�뼱 �꽭�룷 �궡 �뙺�븬 利앷�� �떆�뿉 湲곌났�쓣 �뿴由ш쾶 �븯�뒗 �썝由ш�� �맂�떎.
洹몃┝1. 湲곌났�쓽 援ъ“.
�쇅�뼞�옂 �떇臾쇱쓽 怨듬���꽭�룷�뒗 �빑�씠 �뼇�걹�쑝濡� �띁�졇�엳�뼱 �븘�졊紐⑥뼇�쓣 �씠猷⑤ʼn (A, B, C), �뙇�뼞�옂 �떇臾쇱쓽 怨듬���꽭�룷�뒗 肄⑺뙠紐⑥뼇�쓣 �븯怨� �엳�떎(D, E, F).
A. D. 痢〓㈃�뿉�꽌 蹂� 紐⑥뒿.
B. E. �몴硫댁뿉�꽌 蹂� 湲곌났�옣移섏쓽 紐⑥뒿
C. F. �몴硫댁뿉�꽌 蹂� 紐⑥뒿.
1. 怨듬���꽭�룷 (guard cell)
2. 遺��꽭�룷 (subsidiary cell)
3. 湲곌났�쓽 媛쒗룓 湲곗옉
1) �궙 (鍮쏆씠 �엳�쓣 寃쎌슦)
�몴�뵾�꽭�룷�뱾 以묒뿉�꽌 �쑀�씪�븯寃� �뿽濡앹껜瑜� 吏��땲怨� �엳�뒗 怨듬���꽭�룷�뿉�꽌 愿묓빀�꽦�씠 �닔�뻾�릺�뼱 �뿬�윭 �쑀湲곗궛�뱾�씠 �빀�꽦�릺怨� blue light�뿉 �쓽�빐 �솢�꽦�솕�릺�뒗 H+-�럩�봽�뿉 �쓽�빐 H+ �냽�룄援щ같瑜� �씠猷④쾶 �맂�떎. �씠瑜� 二쇱슂���(�깮�왂)
2) 諛� (鍮쏆씠 �뾾�쓣 寃쎌슦)
�몺 dd-H2O
�몼 0.5 M KCl
�몾 0.5 M NaCl
4. �븫�긽�깭濡� 20遺꾧컙 諛⑹튂�븳 �썑 slide glass �쐞�뿉 臾쇱쓣 �븳諛⑹슱 �뼥�뼱�쑉由� �떎�쓬 �몴�뵾痢듭쓣 援ш꺼吏�吏� �븡寃� slide glass �쐞�뿉 �삱�젮�넃怨� cover glass瑜� �뜮�뒗�떎.
5. �쁽誘멸꼍�븯�뿉�꽌 湲곌났媛쒗룓瑜� 愿�李고븯�뿬 湲곕줉�븳�떎. (���諛곗쑉�뿉�꽌 怨좊같�쑉
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