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1. Introduction
�씠踰� �떎�뿕��� �슚�냼 kinetics�쓽 湲곕낯�씠 �릺�뒗 Michaelis - Menten kinetics �뿉 ����빐�꽌 �씠�빐瑜� �븯怨� Michaelis-Menten equation怨� Lineweaver-Burk equation怨� 媛곴컖�쓽 curve �쓽誘몄�� 李⑥씠�젏�쓣 �떎�뿕寃곌낵瑜� �넻�빐 遺꾩꽍�빐 蹂대뒗 �떎�뿕�씠�떎. �씠瑜� �쐞�빐 吏곸젒 kinetics �뿰援щ�� �쐞�븳 湲곕낯�쟻�씤 �떎�뿕�떒怨꾨�� �닔�뻾�븯�뿬 invertase�쓽 理쒕��諛섏쓳�냽�룄(Vmax)��� Km 媛믪쓣 援ы븯�뿬 鍮꾧탳瑜� �븯寃� �맂�떎. �븘�슱�윭 �슚�냼諛섏쓳�뿉 諛섏쓳�떆媛�, 湲곗쭏�냽�룄媛� 誘몄튂�뒗 �쁺�뼢�쓣 �깮媛곹빐 蹂대뒗�뜲 �쓽�쓽媛� �엳�떎.
��� Michaelis-Menten equation���
Vo = Vmax[S] / ([S] + Km)
Km = [S], when Vo = Vmax/2
��� Lineweaver-Burk equation���
1/Vo = Km/Vmax쨌1/[S] + 1/Vmax
쨌 Vmax - �뿬遺꾩쓽 �슚�냼 �뾾�씠 紐⑤뱺 �슚�냼媛� 諛섏쓳�뿉 李몄뿬�뻽�쓣 �븣�쓽 理쒕�� 諛섏쓳�냽�룄,
�뵲�씪�꽌 �뼱�뼚�븳 �슚�냼�쓽 Vmax媛믪�� 紐⑤몢 媛숇떎.
쨌 Km - Vmax�쓽 1/2�뿉 �룄�떖�븯�뒗�뜲 �븘�슂�븳 湲곗쭏�쓽 �뼇, 洹� 湲곗쭏怨쇱쓽 Affinity瑜� �굹����궡硫�,
�옉�쓣�닔濡� 洹� 湲곗쭏怨쇱쓽 Affinity媛� �겕�떎怨� �븷 �닔 �엳�떎.
2. Materials
5mM Fru, 5mM Glc
0.25M Sucrose
50mM sodium acetate buffer(pH 4.7)
DNS reagent(2.5% DNS in 2M NaOH 4ml + 30% sodium tartarate 6ml + DW 10ml)
invertase(1mg/ml, 50unit/mg, yeast soluble, Sigma)
Heat block, boiling water bath, spectrophotometer, cuvettes
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