항원과 항체

-항체(antibody) : mediator of humoral immunity

1. 항체의 분자구조(molecular structure of antibody)
- monoclonal antibody : permit molecular biological study of antibody molecules

1) 항체분자의 분포 및 순수정제
a) distribution of antibody :
(1) cytoplasmic membrane of B cells which synthesize antibody
(2) plasma of blood and interstitial fluid of tissue
- found in the gamma globulin, immunoglobulin(Ig)
(3) surface of mononuclear phagocytes, NK cells, and mast cells which do not synthesize antibody but have specific receptors for antibody
(4) secretory fluids such as mucus and milk
b) purification
- two step : precipitation using salt (ammonium sulfate) and chromatography