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2) Laboratory evaluation
(1) Semen analysis
- x 3 with consistent period of abstinence (2-5 d.)
- by masturbation into a wide-necked clear glass
- should be analyzed within 1 hr. and kept at body temperature
1> sperm concentration
2> sperm motility
3> sperm morphology
4> fructose
ie) fructose(-) , low volume, & coagulation (-)
① agenesis of vas and seminal vesicle
② ejaculatory duct obstruction
(2) Hormone evaluation
- endocrinologic cause: 3%
- testosterone, LH, FSH, prolactin
- elevated FSH : germinal epithelial damage.
(usually irreversible germ cell damage)
(3) Chromosomal study
(4) Immunologic study
- immunologic cause: 3 -7 %
- SA: normal or spontaneous agglutination, decreased motility
- method;
① sperm agglutination test
② sperm immobilization test
③ immunobead-binding(IBB) technique
(5) Testicular biopsy and vasography
- for Dx. of obstructive azoospermia
(6) Sperm function test
1> sperm-cervical mucus interaction (postcoital test)
2> sperm penetra…
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