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-DNA ̧̉
1. Solution 1
2. Solution 2
3. Solution 3
4. Washing
5. Elution
-PCR
1. PCR tube¿¡ dNTPs buffer 4 ¥ìl¿Í reaction buffer 5 ¥ìl¸¦ ³Ö´Â´Ù.
2. Template DNA 1 ¥ìl¸¦ ³Ö°í forward, reverse primer¸¦ °¢°¢ 0.5 ¥ìl ¾¿ ³Ö´Â´Ù.
3. DNA polymerase 1 ¥ìl¸¦ ³Ö´Â´Ù.
4. Áõ·ù¼ö 37 ¥ìl¸¦ ³Ö°í Àß pipetting ÇØ ÁØ´Ù.
5. PCR machineÀÇ well¿¡ tube¸¦ ³Ö°í ¹ÝÀÀ½ÃŲ´Ù.
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-PCR
DNA polymerase, Template DNA, primers, dNTPs mixture, Reaction buffer, PCR tube, Áõ·ù¼ö, pipette tip, pipette
`miniprepÀÇ ¿ø¸®`
Plasmid DNA extraction
Plasmid¸¦ ºÐ¸®Çس»´Â ¹æ¹ýÀº ÃßÃâ·®¿¡ µû¶ó miniprep, midiprep, maxiprep ÀÌ ÀÖ´Ù.
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