|
_SLIDE_1_
�쟾湲곗쁺�룞(Electrophoresis)
_SLIDE_2_
�쟾湲곗쁺�룞(Electrophoresis)
�씪諛섏쟻�씤 �떎�뿕�떎�뿉�꽌�쓽 �쟾湲곗쁺�룞��� DNA�굹 �떒諛깆쭏�쓣 �겕湲곗뿉 �뵲�씪 遺꾪쉷�븯湲� �쐞�븳 諛⑸쾿 以� �븯�굹濡쒖뜥, DNA��� �떒諛깆쭏��� �쟾�븯瑜� �씈怨� �엳湲� �븣臾몄뿉 �쟾湲곕�� 嫄몄뼱二쇰㈃ 媛� 遺꾩옄�쓽 �쟾湲곗쟻 �듅�꽦�뿉 �뵲�씪 �씠�룞�븯寃� �맂�떎
�쟾�븯瑜� �씍 遺꾩옄�솕�빀臾쇱뿉 �씪�젙�븳 �쟾�븬�쓣 嫄몄뼱二쇱뼱 遺꾩옄�웾, �쟾�븯�웾�벑�쓽 臾쇰━�솕�븰�쟻 �꽦吏덉쓽 李⑥씠�뿉 �뵲�씪 遺꾨━�떆�궎�뒗 諛⑸쾿�쑝濡�, 留ㅼ쭏�쓽 醫낅쪟�뿉 �뵲�씪 agarose, polyacrylamide, starch gel �쟾湲곗쁺�룞�쑝濡� 援щ퀎
DNA�쓽 寃쎌슦�뿉�뒗 �씤�궛湲곌�� �쓬�쟾�븯瑜� �씈湲� �븣臾몄뿉 �뼇洹뱀そ�쑝濡� �씠�룞�쓣 �븳�떎
_SLIDE_3_
DNA �솗�씤諛⑸쾿
�삎愿묒꽦 �떆�빟�씤 Ethidium bromide(EtBr)�쓣 泥섎━�븯寃� �릺硫�, �씠 �떆�빟�씠 �젮�쓽 DNA�뿉 �겮�뼱�뱾�뼱媛먯쑝濡쒖뜥, �옄�쇅�꽑 �븯�뿉�꽌 DNA band瑜� �솗�씤�븷 �닔 �엳�떎
`�떎�뼇�븳 range�쓽 DNA marker`
_SLIDE_4_
DNA�쓽 �씠�룞�냽�룄�뿉 �쁺�뼢�쓣 二쇰뒗�슂�냼�뱾
DNA 遺꾩옄�쓽 �겕湲�
- DNA 遺꾩옄媛� �겢�닔濡� �뒓由ш퀬 �씠�룞�븯怨�, �옉�쓣�닔濡� 鍮⑤━ �씠�룞
�쟾湲곗쁺�룞 �셿異⑹슜�븸�쓽 �꽦遺꾧낵 �씠�삩媛뺣룄
- �씠�삩媛뺣룄媛� �궙�쑝硫� DNA媛� �뒓由ш쾶 �씠�룞
Agarose �냽�룄
- Agarose �냽�룄媛� �넂�쓣�닔濡� �뒓由ш쾶 �씠�룞
DNA �삎�깭
- Supercoiled DNA媛� 媛��옣 鍮⑤━, 洹� �떎�쓬 linear DNA, open circular DNA �닚�쑝濡� �씠�룞
�쟾�븬
- 遺��븯�릺�뒗 �쟾�븬�씠 �넂�쓣�닔濡� 鍮좊Ⅴ寃� �씠�룞
_SLIDE_5_
�떎�뿕 Method
Sample loading�븯湲�
�몺 gel ����쓣 1X TAE buffer瑜� 梨꾩슫 �쟾湲곗쁺�룞 �옣移섏뿉 �떞洹쇰떎
�몼 comb�뿉 buffer媛� 李⑤룄濡� �빐二쇰ʼn comb�뿉 媛�源뚯슫 諛⑺뼢�씠 (-)履쎌쑝濡� �뼢�븯寃� �븳�떎
�몾 DNA sample�뿉 dye瑜� �꽎�뼱以��떎
�몿 泥� 踰덉㎏ comb�뿉 marker瑜� loading�븳�떎
�뫀 �씠�썑濡� DNA sample�쓣 ���(�깮�왂)
|
인 쇄
바로가기
�쟾湲곗쁺�룞.pptx
[Size :
968 Kbyte
]
장바구니
