실험보고서- 유기화학 실험[TLC]

REPORT OF LABORATORY EXPERIMENTS IN ORGANIC CHEMISTRY ①

1. Subject : Thin Layer Chromatography (TLC)
2. Date :
3. Name : -
4. Principle :
Non-volatile reaction mixture의 qualitative examination에 사용이 되는 simple하고 rapid한 일종의 liquid chromatograghy로 glass, aluminum 또는 plastic plate에 solid stationary phase가 coating되어있다.
원리는 분리하고자 하는 물질의 mobile phase와 stationary phase에 대한 adsorption정도에 따라 물질이 분리된다.
(1) 사용되는 경우
- Sample purification monitoring
- Quick establishment of optimum conditions for carrying out a synthesis
- Examination of crude reaction mixture
- Determining the homogeneity of a sample
- Conjunction with other evidence
- Establishing whether or not samples from different sources are identical
⇒ Qualitative analysis of quantities as small as 10 microgram
(2) Adsorbent (Stationary phases)
- Silica gel, alumina, kieselguhr, cellulose powder
- Fluorescent compound에 사용이 가능하며 mixture compound의 resolut…