-Date
-Title
¨çDNA Isolation
¨èPCR(polymerase chain reaction)
¨éÀü±â¿µµ¿(electrophoresis)
-Object
¨ç¹ÚÅ׸®¾Æ·ÎºÎÅÍ DNA¸¦ ºÐ¸®ÇØ ³½´Ù.
¨èƯÁ¤ DNA ºÎÀ§¸¦ ¹Ýº¹ ÇÕ¼ºÇÏ¿© ¿øÇÏ´Â DNA ºÐÀÚ¸¦ ÁõÆø ½ÃŲ´Ù.
¨éÀü±â¿µµ¿Àº DNA¸¦ Å©±â¿¡ µû¶ó¼ ºÐ¸®ÇÏ´Â ¹æ¹ýÀÌ´Ù.
-Materials&
Methods
¨çCell lysis sol, proteinase K, RNase, PPT sol, e-tube, DNA binding sol, spin colmn, column washing sol, water, ¿ø½ÉºÐ¸®±â
1. Cell lysis sol 300§¡ (inverting)
2. proteinase K 10§¡ (inverting) 56¡É¿¡¼ 15ºÐ
3. RNase A 3§¡ 37¡É¿¡¼ 15ºÐ
4. PPT sol 100§¡¡æ Vortexing¡æ In ice¿¡¼ 5ºÐ
5. 10ºÐ µ¿¾È 1300rpm centrifuge
6. »óµî¾× 390§¡¡æ e-tube¡æ DNA binding sol 600§¡
7. 650§¡ in Spin column¡æ 1ºÐ µ¿¾Ècentrifuge
8. Column washing sol 600§¡¡æ 1ºÐ µ¿¾Ècentrifuge
9. Column washing sol 500§¡¡æ 1ºÐ µ¿¾Ècentrifuge
10. spin columnÀ» »õ e tube¿¡ ¿Å±â°í water 100§¡¸¦ ³Ö°í 5ºÐ Á¤Áö ÈÄ 1ºÐ °£ centrifuge.
¨èDNA polymerase, Template DNA, primers, dNTPs, mixture, reaction buffer, PCR tube, Áõ·ù¼ö, pipette ¡¦(»ý·«)
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