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0. ÃÊ·Ï
1. ¼ ·Ð
1.1 Å©·Î¸¶Åä±×·¡ÇÇ(Chromatography)
1.1.1 Å©·Î¸¶Åä±×·¡ÇÇÀÇ ºÐ·ù(Á¾·ù)
1.2 °í¼º´É ¾×ü Å©·Î¸¶Åä±×·¡ÇÇ(High Performance Liquid Chromatography, HPLC)
1.2.1 HPLCÀÇ ±âº» ±¸¼º¿ä¼Ò
1.2.2 HPLCÀÇ column efficiency
1.2.3 internal/external standard
1.2.4 isocratic/gradient elution
2. ½ÇÇè¹æ¹ý
2.1 »ç¿ëÇÑ ½Ã¾à ¹× ±â±¸
2.2 Construct standard curves using chromatogram of analytes
2.3 ÁÖÀÇ»çÇ×
3. °á°ú ¹× °íÂû
4. °á ·Ð
Reference
º»¹®/³»¿ë
1. ¼ ·Ð
1.1 Å©·Î¸¶Åä±×·¡ÇÇ(Chromatography)
Å©·Î¸¶Åä±×·¡ÇÇ´Â chromos(color)¿Í graphein(write)ÀÇ ÇÕ¼º¾î, Áï »öÀ» ÅëÇØ ºÐ¼®ÇÏ´Â °ÍÀ¸·Î, È¥ÇÕ¹°(mixture)ÀÇ ºÐ¸® ¹æ¹ý Áß ÇϳªÀÌ´Ù. ´Ù¸¥ È¥ÇÕ¹° ºÐ¸® ¹æ¹ý Áß ÇϳªÀÎ ÃßÃâ(extraction)°ú °°Àº ¿ø¸®·Î ½ÃÇàµÇÁö¸¸, ÇϳªÀÇ »ó(phase)Àº ´Ù¸¥ ÇϳªÀÇ »óÀÌ Áö³ª°¡´Â µ¿¾È °íÁ¤µÈ´Ù´Â Á¡¿¡¼ ´Ù¸£´Ù. ÀÌ ¶§ Ä®·³(column)À» µû¶ó ¿òÁ÷ÀÌ´Â ¿ë¸Å(solvent)¸¦ À̵¿»ó(mobile phase)À̶ó°í Çϴµ¥, ¾×ü
¶Ç´Â ±âüÀÌ´Ù. ¹Ý¸é¿¡ Ä®·³ ¼Ó¿¡¼ ¸Ó¹«¸£´Â »óÀ» °íÁ¤»ó(stationary phase)À̶ó°í Çϸç, ´ëºÎºÐ °ü¼Ó¿¡ ä¿öÁø °íü ÀÔÀÚµéÀÇ Ç¥¸é ȤÀº ¸ð¼¼°üÀÇ ³»ºÎ¿¡ ÈÇÐÀûÀ¸·Î °áÇÕµÈ Á¡¼º(viscous) ¾×üÀÌ´Ù.
1.1.1 Å©·Î¸¶Åä±×·¡ÇÇÀÇ ºÐ·ù(Á¾·ù)
°íÁ¤»óÀ» Æ÷ÇÔÇÑ ½ÇÇè±â±¸ÀÇ ÇüŰ¡ Æò¸éÀÎ °æ¿ì Æò¸é Å©·Î¸¶Åä±×·¡ÇÇÀ̸ç, ÀÌ´Â ´Ù½Ã Á¾ÀÌ Å©·Î¸¶Åä±×·¡ÇÇ(paper chromatography, PC)¿Í ¹ÚÃþ Å©·Î¸¶Åä±×·¡ÇÇ(thin layer chromatography, TLC)·Î ³ª´¶´Ù. °íÁ¤»óÀ» Æ÷ÇÔÇÑ ½ÇÇè±â±¸ÀÇ ÇüŰ¡ Ä®·³ÀÎ °æ¿ì Ä®·³ Å©·Î¸¶Åä±×·¡ÇÇÀÌ´Ù.
À̵¿»óÀÌ ¾×üÀÎ °æ¿ì ¾×ü Å©·Î¸¶Åä±×·¡ÇÇ(liquid chromatography, LC), ±âüÀÎ °æ¿ì ±âü Å©·Î¸¶¡¦(»ý·«)
Âü°í¹®Çå
Daniel C. Harris, Quantitative Chemical Analysis(8th ed.), W.H. Freeman and Company, pp. 542-559(2010)
Sawyer. D. T. Heineman, W. R. Beebe, J. M., Chemistry Experiments for Instrumental Methods, John Wiley & Sons, pp. 344-353(1984)
K. Tsuji, J. H. Robertson, and W. F. Beyer, ¡°Analytical Chemistry¡±, 46, p. 539 (1974)
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