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◦ Betts DH, Bordignon V, Smith L, Semple E, King WA. Telomerase activity in bovine blastocysts, fetal fibroblast and stem cell-like cell lines cultured under various conditions. Theriogenology, 51:181, 1999.
◦ Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA, Robl JM. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science, 280:1256-1258, 1998.
◦ Fulka Jr. J, First NL, Loi P, Moor RM. Cloning by somatic cell nuclear transfer. BioEssays, 20:847-851, 1998.
◦ McGrath J, Solter D. Nuclear transplantation in the mouse embryo by microsurgery and cell fusion. Science, 220:1300-1302, 1983.
◦ Wakayama T, Perry ACF, Zuccotti M, Johnson KR, Yanagimachi R. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394:369-373, 1998.
◦ Wells DN, Misica PM, McMillan WH, Tervit HR. Production of cloned bovine fetuses following nuclear transfer using cells from a fetal fibroblast cell line. Theriogenology, 49:330 abst., 1998
◦ Willadsen SM. Nuclear transplantation in sheep embryos. Nature, 320:63-65, 1986.
◦ Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable dffspring derived from fetal and adult mammalian cells. Nature, 385:810-813, 1997.