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[°øÇÐ]½ÇÇ躸°í¼ - Plasmid ºÐ¸® ½ÇÇè & Agarose gelÀ» ÀÌ¿ëÇÑ plasmid Àü±â¿µµ¿ / Plasmid ºÐ¸® ½ÇÇè & Agarose gelÀ» ÀÌ¿ëÇÑ plasmid Àü±â¿µµ¿ 1. Purpose E.coli¸¦ ¹è¾çÇØ Çö󽺹̵带 ºÐ¸®ÇÏ°í, ºÐ¸®ÇÑ Çö󽺹̵带 Á÷Á¢ ¸¸µç Agarose gelÀ» ÀÌ¿ëÇÏ¿© Àü±â ¿µµ¿ÇÑ´Ù. 2. Introduction Plasmid DNA ºÐ¸®ÀÇ ¿ø¸® ÁÖ·Î mini-prepÀ̶ó°í ºÎ¸£´Â ÀÌ ¹æ¹ýÀº plasmid DNA¸¦ ¼Ò·® ºÐ¡¦ |
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¡¥/ 1. Purpose 2. Theory 1) Plasmid DNA extraction 3. Methods 1) Chemicals & Apparatus 2) Procedure 4. Data & Results 1) Alkaline Lysis 2) Purification 3) Concentration measurement 4) Agatose Gel Electrophoresis 5. Discussion / [½æ³×ÀÏÀ» È®ÀÎÇØ ÁÖ¼¼¿ä] |
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Plasmid DNA isolation from bacterial cell Miniprep ¿¹ºñ·¹Æ÷Æ® [A+] / ¿¹ºñ·¹Æ÷Æ® ½ÇÇèÁ¦¸ñ : Plasmid DNA isolation from bacterial cell : Miniprep Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. ½ÇÇè ¸ñÀû - DNA isolation ¹æ¹ý ÁßÀÇ ÇϳªÀÎ MiniprepÀ» ÀÌ¿ëÇÏ¿© ¾ËÄ®¸® Á¶°Ç ÇÏ¿¡¼ ¹Ì»ý¹°(´ëÀå±Õ)ÀÇ ¼¼Æ÷º®°ú ¼¼Æ÷¸·À» ºÎ¼ö°í À¯ÀüÀÚ ÀçÁ¶ÇÕ¿¡ ÇÊ¿äÇÑ Plasmid DNA¸¦ ºÐ¸®ÇÑ´Ù. 2. ¹ÙÅÁ ÀÌ·Ð (¡¦ |
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Plasmid DNA isolation from bacterial cell Miniprep °á°ú·¹Æ÷Æ® [A+] / °á°ú·¹Æ÷Æ® ½Ç Çè Á¦ ¸ñ : Plasmid DNA isolation from bacterial cell : Miniprep Á¶ : ÇÐ ¹ø : ÀÌ ¸§ : 1. Abstract À̹ø ½ÇÇèÀÇ ¸ñÇ¥´Â DNA isolation ¹æ¹ý ÁßÀÇ ÇϳªÀÎ MiniprepÀ» ÀÌ¿ëÇÏ¿© ¾ËÄ®¸® Á¶°Ç ÇÏ¿¡¼ ¹Ì»ý¹°(´ëÀå±Õ)ÀÇ ¼¼Æ÷º®°ú ¼¼Æ÷¸·À» ºÎ¼ö°í À¯ÀüÀÚ ÀçÁ¶ÇÕ¿¡ ÇÊ¿äÇÑ Plasmid DNA¸¦ ºÐ¸®Çϴ¡¦ |
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PCR°ú Gel runningÀ» ÀÌ¿ëÇØ ´ëÀå±ÕÀÇ plasmid °üÂû½ÇÇè / DNA technology Abstract »ý¸íüÀÇ À¯ÀüÁ¤º¸¸¦ ´ã°í ÀÖ´Â DNA´Â ±× Á߿伺À» ÀÎÁ¤¹Þ¾Æ 21¼¼±â À¯Àü°øÇÐ ¿¬±¸¿¡ ÀÖ¾î ¿¬±¸°¡ ¸¹ÀÌ ÁøÇàµÈ ´ë»ó Áß ÇϳªÀÌ´Ù. ±×·¡¼ À̹ø module 3¿¡¼± DNA ¿¬±¸¿¡ ÀÖ¾î ÈçÇÏ°Ô »ç¿ëµÇ´Â DNA cloning, colony PCR, gel running ±â¹ý¿¡ ´ëÇØ ¾Ë¾Æº¸°íÀÚ ÇÑ´Ù. À̸¦ À§ÇØ ´ëÀå±ÕÀ» competent cell·Î¡¦ |
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[°øÇÐ,±â¼ú] ½ÇÇ躸°í¼ - Plasmid DNA Purification / Plasmid DNA Purification 1. ¼·Ð Plasmid´Â bacteria ¼¼Æ÷ÀÇ ¿°»öü¿Í´Â º°µµ·Î Á¸ÀçÇÏ¸é¼ self-replicationÀ» ÇÒ ¼ö ÀÖ´Â ¿øÇü DNA ºÐÀÚ·Î ÀûÀº ¼öÀÇ geneÀ» °¡Áö°í ÀÖ´Ù. ÀÌ plasmid´Â À¯ÀüÀÚ ÀçÁ¶ÇÕ ½Ã ÀçÁ¶ÇÕ DNA Á¶ÀÛÀ» À§ÇÑ vector DNA(¼÷ÁÖ¿¡ ÀÌÁ¾ÀÇ DNA¸¦ ¿î¹ÝÇÏ´Â DNA)·Î »ç¿ëµÇ¸ç ¿øÇÏ´Â DNA ´ÜÆíÀ» ³Ö¾î gene cloni¡¦ |
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[»ý¸í°øÇÐ Plasmid DNA preparation / (1) ½ÇÇè Á¦¸ñ : Plasmid DNA preparation (2) ½ÇÇè ¸ñÀû : Ampicillin¿¡ ´ëÇÏ¿© ÀúÇ×¼ºÀ» Áö´Ñ E. coli (XLI-Blue, Amp-r) ÀÇ plasmid DNA¡¼pBlue½ºÅ©¸³Æ® SK¥±(+)¡½¸¦ isolation ÇÑ´Ù. (3) ½ÇÇè ¿ø¸® : Plasmid DNA¸¦ Á¤Á¦Çϱâ À§Çؼ ¿©·¯ °¡Áö ¹æ¹ýµéÀÌ »ç¿ëµÇ´Âµ¥ °øÅëÀûÀ¸·Î ´ÙÀ½ÀÇ ¼¼ °úÁ¤- ¹Ì»ý¹°ÀÇ ¹è¾ç°ú Çö󽺹̵å ÁõÆø ; ¹Ì»ý¹°¡¦ |
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[ÀÚ¿¬°úÇÐ] ¹Ì»ý¹°ÇÐ ½ÇÇè - Mini-Prep[Hybrid-Q Plasmid Rapid prep Kit]½ÇÇè / Á¦¸ñ: Mini-Prep (Hybrid-Q Plasmid Rapid prep Kit) 1.½ÇÇè¸ñÀû Mini PrepÀ̶õ DNA-isolation¿¡ ÀÖ´Â ¿ø¸® Áß Çϳª·Î Plasmid DNAºÐ¸®¿¡ »ç¿ëµÇ´Â ¹æ¹ýÀÌ¸ç ¸¹Àº È常¦ ºü¸¥ ½Ã°£ ³»¿¡ ºÐ¼®ÇÏ´Â ¹æ¹ýÀÌ´Ù. PrepÀº PrepartionÀÇ ¾àÀÚ.DNA¸¦ ¼Ò·®À¸·Î ÃßÃâÇÑ´Ù´Â ÀǹÌ. ÁÖ·Î ÀÌ¿ëµÇ´Â Alkylation lysis¡¦ |
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¡¥romosomal DNA¸¦ ÃßÃâÇÏ°í, E.Coli ÀÇ Plasmid¸¦ ÃßÃâÇؼ Á¦ÇÑÈ¿¼Ò 󸮸¦ ÅëÇØ plasmid »óŸ¦ È®ÀÎÇÔÀ¸·Î½á DNA¸¦ ´Ù·ç´Â ¹æ¹ýÀ» ÀÍÇû´Ù. Chromosomal DNA Extraction ¼¼±ÕÀ¸·ÎºÎÅÍ DNA¸¦ ÃßÃâÇÏ´Â ¹æ¹ýÀÌ´Ù. ¼¼±Õ¿¡ SDS(¼¼Á¦)¸¦ °¡ÇÏ°í ¿Âµµ¸¦ ³ôÀÌ¸é ¼¼±ÕÀÇ ¼¼Æ÷º®¿¡ ºÙ¾î ÀÖ´ø ÀÎÁöÁúÀÌ ¿ëÇصǾî DNA¸¦ ¼¼Æ÷·ÎºÎÅÍ Çظ®½ÃŲ´Ù. DNA¿ë¾×¿¡ ¿¡Åº¿ÃÀ» °¡Çϸé DNA´Â ¾ËÄڿÿ¡ ´ëÇØ ºÒ¡¦ |
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[ÀÚ¿¬°úÇÐ] ºÐÀڹ̻ý¹°ÇÐ ½ÇÇè - mini preparation(plasmid DNA¸¦ ¼Ò·® ºÐ¸®ÇÏ´Â ¹æ¹ý) ½ÇÇè / title mini preparation object DNAÀÇ Å©±âÂ÷ÀÌ¿¡ ÀÇÇØ ºÐ¸®ÇÏ´Â ¹æ¹ýÀÎ alkaline lysis method¸¦ ÀÌ¿ëÇÏ¿© Á¤Á¦µÈ DNAÀÇ ¾çÀ» ºÒ¸°´Ù. introduction À¯ÀüÀÚ ÀçÁ¶ÇÕÀº DNA¸¦ ¸ñÀû¿¡ µû¶ó ÀûÀýÈ÷ ÀÚ¸£°í ºÙ¿©¼ À¯Àü¹°ÁúÀ» ¿øÇÏ´Â ÇüÅ·Π°³Á¶½ÃÅ°´Â °úÁ¤ÀÌ´Ù. °¡Àå ±âº»ÀûÀÎ DNAÁ¶À۱⡦ |
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