³«³ó¹Ì»ý¹°ÇÐ ¹× ½ÇÇè-Use of selective(ȹ¼±ÆòÆǹý) & differential Media(ºÐº°¹èÁö)
1. Introduction
-À̹ø ½ÇÇèÀº Streaking(ȹ¼±ÆòÆǹý)À¸·Î Selective media(¼±ÅùèÁö)¿Í Differential media(ºÐº°¹èÁö)¿¡¼ ½Ã·á¾È¿¡ Àִ ƯÁ¤ ¹Ì»ý¹°À» ¼ø¼ö ºÐ¸®ÇÏ´Â °ÍÀÌ´Ù.
-Streaking(ȹ¼±ÆòÆǹý)À̶õ °íü¹èÁö(agar)¿¡ ¹é±ÝÀÌ·Î ±ÕÀ» ºÐÁÖÇÏ°í ¹è¾çÇÏ¿© ´ÜÀϱÕÁÖÁý¶ô(single colony)¸¦ ¾ò´Â ¹æ¹ýÀÌ´Ù.
-¹èÁö(media)¿¡´Â ¹Ì»ý¹°ÀÇ »ýÀå¿¡ ÇÊ¿äÇÑ ¿µ¾ç¹°ÁúÀ» Æ÷ÇÔÇÏ°í ÀÖ´Ù. ¹èÁö¿¡´Â Å©°Ô ¾×ü¹èÁö(Broth)¿Í ¾×ü¹èÁö¿¡ ÇÑõÀ» ÷°¡ÇÏ¿© ±»Èù °íü¹èÁö(Agar)°¡ ÀÖ´Ù.
-Selective media(¼±ÅùèÁö)´Â ¼±ÅÃÇÑ Æ¯Á¤ ¹Ì»ý¹°ÀÇ »ýÀ°À» ÁõÁø½ÃÅ°°í ´Ù¸¥ ¹Ì»ý¹°ÀÇ »ýÀ°ÀÌ ¾ïÁ¦µÇµµ·Ï ¸¸µé¾îÁø ¹èÁöÀÌ´Ù.
-Differential media(ºÐº°¹èÁö)´Â »ýÀåÀ̳ª ´ë»ç¹°ÀÇ Â÷ÀÌ¿¡ ±âÃÊÇÏ¿© ¼·Î ´Ù¸¥ ¹Ì»ý¹°À» ±¸º°Çϱâ À§ÇØ »ç¿ëµÇ´Â ¹èÁöÀÌ´Ù.
2. Materials
1) reagent
Selective media - LB(Luria-Bertani) agar + Ampicillin, LB agar, MRS-maltose, Differential media - MRS-sucrose, TDCA, BCP agar
2) apparatus
¹é¡¦(»ý·«)
3) sample
3. Methods
4. µÎ ¹ø° Æ۶߸° ±ÕÀ» Á¶±Ý µû¼ °íü¹èÁö ºó °ø°£¿¡ Æ۶߸°´Ù.
5. Discussion
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