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1. Title : RT-PCR
2. Objective : poliovirus�쓽 RNA瑜� RT-PCR�쓣 �씠�슜�빐 cDNA濡� 留뚮뱺 �뮘, PCR�쓣 �씠�슜�빐 利앺룺�떆�궓 �썑 �쟾湲� �쁺�룞�븯�뿬 �겕湲곕�� 愿�李고븳�떎.
3. Principle : poliovirus�쓽 RNA�뿉 �듅�씠�쟻�쑝濡� 寃고빀�븯�뒗 �봽�씪�씠癒몃�� �씠�슜�븯�뿬 poliovirus�쓽 cDNA留뚯쓣 留뚮뱾 �닔 �엳�쑝硫� �쟾湲곗쁺�룞�쑝濡� 諛대뱶瑜� 愿�李고븳�떎.
4. Method
1) RT-PCR : cDNA�깮�꽦
RT-PCR��� �샎�빀�떆�궓 premix瑜� �궗�슜. premix�뿉�뒗 taq pol, dNTP, PCR 理쒖쟻�쓽 buffer媛� �뱾�뼱媛� �엳�떎. premix��� RNA 二쇳삎�쓣 5ul, primer 3ul, RNA free water 13ul瑜� �꽔�뼱以��떎.
�몺 72�꼦�뿉�꽌 5遺�
�몼 42�꼦�뿉�꽌 60遺�
�몾 94�꼦�뿉�꽌 5遺꾧컙
(以묐왂)
6. Discussion
以묓빀�슚�냼��� �뿰�뇙諛섏쓳踰뺢낵 �뿭�쟾�궗�슚�냼 諛섏쓳�쓣 �샎�빀�븳 RT-PCR(reverse tran�뒪�겕由쏀듃ase -polymerase chain reaction)踰뺤�� �꽭�룷�뿉�꽌 異붿텧�븳 mRNA�뿉�꽌 cDNA瑜� �빀�꽦�븯怨� �씠寃껋쓣 二쇳삎�쑝濡� �븯�뿬 PCR濡� 利앺룺�븳 �썑 遺꾩꽍�븯�뒗 湲곗닠�씠�떎. �씠 諛⑸쾿�쑝濡� mRNA 醫낅쪟瑜� �솗�씤�븯怨� �쑀�쟾�옄 諛쒗쁽 �닔以��쓣 �븣�븘�궪 �닔 �엳�떎.
(以묐왂)
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